ACS_sensors
July 28
2023Volume 8
Issue 7Pages 2412-2895
Ultrasensitive Determination of Trypsin in Human Urine Based on Amplified Fluorescence Response
The determination of trypsin activity in human urine is important for evaluating pancreatic disease. We designed an effective fluorescence sensing strategy based on a self-assembled amphiphilic pyrene/protamine complex system that provides an amplified fluorescence response for highly sensitive and selective detection of trypsin. In aqueous solution, the functionalized pyrene formed fluorescent, π-extended aggregates inside micelles, which were effectively quenched by protamine (a trypsin substrate). However, this quenched fluorescence was very sensitively recovered by the trypsin’s enzymatic reaction, and this was attributed to a marked reduction in enhanced exciton migration caused by protamine in π-delocalized pyrene aggregates. The devised sensing platform was successfully utilized to selectively and sensitively detect trypsin at very low concentrations (0.03–0.5 μg mL–1) in non-pretreated human urine and to screen for trypsin inhibitors at concentrations of 0.1–5.0 μg mL–1.
- Taemin Park
- Minwoo Han
- Kirk S. Schanze
- Seoung Ho Lee
https://pubs.acs.org/doi/10.1021/acssensors.3c00297
Image created by minjeong Kim / Nanosphere
ACS_sensors
July 28
2023Volume 8
Issue 7Pages 2412-2895
Ultrasensitive Determination of Trypsin in Human Urine Based on Amplified Fluorescence Response
The determination of trypsin activity in human urine is important for evaluating pancreatic disease. We designed an effective fluorescence sensing strategy based on a self-assembled amphiphilic pyrene/protamine complex system that provides an amplified fluorescence response for highly sensitive and selective detection of trypsin. In aqueous solution, the functionalized pyrene formed fluorescent, π-extended aggregates inside micelles, which were effectively quenched by protamine (a trypsin substrate). However, this quenched fluorescence was very sensitively recovered by the trypsin’s enzymatic reaction, and this was attributed to a marked reduction in enhanced exciton migration caused by protamine in π-delocalized pyrene aggregates. The devised sensing platform was successfully utilized to selectively and sensitively detect trypsin at very low concentrations (0.03–0.5 μg mL–1) in non-pretreated human urine and to screen for trypsin inhibitors at concentrations of 0.1–5.0 μg mL–1.
https://pubs.acs.org/doi/10.1021/acssensors.3c00297
Image created by minjeong Kim / Nanosphere